Sequencing:Article Title: Human CD4 + T cells recognize Mycobacterium tuberculosis –infected macrophages amid broader responses
Article Snippet: All TCRs were engineered as hybrid TCRs, in which the entire human α and β V(D)J regions were preserved, and these nucleotides were grafted onto mutant mouse α and β constant regions, respectively, and cloned into the pDONR221 Entry vector as described previously ( ). .. The codon-optimized nucleotide sequence for hybrid αβTCR sequences was synthesized (Twist Biosciences) as a single polyprotein separated by a 2A self-cleaving peptide sequence (TCRβ-P2A-TCRα) and subsequently cloned into the pDONR22 entry vector and then transferred into the pHAGE-EF1:DEST-PGK:CD4 destination vector provided by Dr. Mohammad Haj Dezfullian (University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA), using LR Clonase (Invitrogen) according to the Gateway cloning methodology (Invitrogen) and as described previously , and used to transduce 2T1R-competent cells (Invitrogen). .. The codon-optimized nucleotide sequence for hybrid αβTCR sequences was synthesized (Twist Biosciences) as a single polyprotein separated by a 2A self-cleaving peptide sequence (TCRβ-P2A-TCRα) and subsequently cloned into the pDONR22 entry vector and then transferred into the pHAGE-EF1:DEST-PGK:CD4 destination vector provided by Dr. Mohammad Haj Dezfullian (University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA), using LR Clonase (Invitrogen) according to the Gateway cloning methodology (Invitrogen) and as described previously , and used to transduce 2T1R-competent cells (Invitrogen).
Article Title: Human CD4+ T cells recognize Mycobacterium tuberculosis-infected macrophages amid broader responses.
Article Snippet: .. The codon-optimized nucleotide sequence for hybrid αβTCR sequences was synthesized (Twist Biosciences) as a single polyprotein separated by a 2A self-cleaving peptide sequence (TCRβ-P2A-TCRα) and subsequently cloned into the pDONR22 entry vector and then transferred into the pHAGE-EF1:DEST-PGK: CD4 destination vector provided by Dr. Mohammad Haj Dezfullian (University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA), using LR Clonase (Invitrogen) according to the Gateway cloning methodology (Invitrogen) and as described previously (Dezfulian et al., 2023), and used to transduce 2T1R-competent cells (Invitrogen). .. The codon-optimized nucleotide sequence for hybrid αβTCR sequences was synthesized (Twist Biosciences) as a single polyprotein separated by a 2A self-cleaving peptide sequence (TCRβ-P2A-TCRα) and subsequently cloned into the pDONR22 entry vector and then transferred into the pHAGE-EF1:DEST-PGK: CD4 destination vector provided by Dr. Mohammad Haj Dezfullian (University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA), using LR Clonase (Invitrogen) according to the Gateway cloning methodology (Invitrogen) and as described previously (Dezfulian et al., 2023), and used to transduce 2T1R-competent cells (Invitrogen).
Synthesized:Article Title: Human CD4 + T cells recognize Mycobacterium tuberculosis –infected macrophages amid broader responses
Article Snippet: All TCRs were engineered as hybrid TCRs, in which the entire human α and β V(D)J regions were preserved, and these nucleotides were grafted onto mutant mouse α and β constant regions, respectively, and cloned into the pDONR221 Entry vector as described previously ( ). .. The codon-optimized nucleotide sequence for hybrid αβTCR sequences was synthesized (Twist Biosciences) as a single polyprotein separated by a 2A self-cleaving peptide sequence (TCRβ-P2A-TCRα) and subsequently cloned into the pDONR22 entry vector and then transferred into the pHAGE-EF1:DEST-PGK:CD4 destination vector provided by Dr. Mohammad Haj Dezfullian (University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA), using LR Clonase (Invitrogen) according to the Gateway cloning methodology (Invitrogen) and as described previously , and used to transduce 2T1R-competent cells (Invitrogen). .. The codon-optimized nucleotide sequence for hybrid αβTCR sequences was synthesized (Twist Biosciences) as a single polyprotein separated by a 2A self-cleaving peptide sequence (TCRβ-P2A-TCRα) and subsequently cloned into the pDONR22 entry vector and then transferred into the pHAGE-EF1:DEST-PGK:CD4 destination vector provided by Dr. Mohammad Haj Dezfullian (University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA), using LR Clonase (Invitrogen) according to the Gateway cloning methodology (Invitrogen) and as described previously , and used to transduce 2T1R-competent cells (Invitrogen).
Article Title: Human CD4+ T cells recognize Mycobacterium tuberculosis-infected macrophages amid broader responses.
Article Snippet: .. The codon-optimized nucleotide sequence for hybrid αβTCR sequences was synthesized (Twist Biosciences) as a single polyprotein separated by a 2A self-cleaving peptide sequence (TCRβ-P2A-TCRα) and subsequently cloned into the pDONR22 entry vector and then transferred into the pHAGE-EF1:DEST-PGK: CD4 destination vector provided by Dr. Mohammad Haj Dezfullian (University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA), using LR Clonase (Invitrogen) according to the Gateway cloning methodology (Invitrogen) and as described previously (Dezfulian et al., 2023), and used to transduce 2T1R-competent cells (Invitrogen). .. The codon-optimized nucleotide sequence for hybrid αβTCR sequences was synthesized (Twist Biosciences) as a single polyprotein separated by a 2A self-cleaving peptide sequence (TCRβ-P2A-TCRα) and subsequently cloned into the pDONR22 entry vector and then transferred into the pHAGE-EF1:DEST-PGK: CD4 destination vector provided by Dr. Mohammad Haj Dezfullian (University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA), using LR Clonase (Invitrogen) according to the Gateway cloning methodology (Invitrogen) and as described previously (Dezfulian et al., 2023), and used to transduce 2T1R-competent cells (Invitrogen).
Clone Assay:Article Title: Human CD4 + T cells recognize Mycobacterium tuberculosis –infected macrophages amid broader responses
Article Snippet: All TCRs were engineered as hybrid TCRs, in which the entire human α and β V(D)J regions were preserved, and these nucleotides were grafted onto mutant mouse α and β constant regions, respectively, and cloned into the pDONR221 Entry vector as described previously ( ). .. The codon-optimized nucleotide sequence for hybrid αβTCR sequences was synthesized (Twist Biosciences) as a single polyprotein separated by a 2A self-cleaving peptide sequence (TCRβ-P2A-TCRα) and subsequently cloned into the pDONR22 entry vector and then transferred into the pHAGE-EF1:DEST-PGK:CD4 destination vector provided by Dr. Mohammad Haj Dezfullian (University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA), using LR Clonase (Invitrogen) according to the Gateway cloning methodology (Invitrogen) and as described previously , and used to transduce 2T1R-competent cells (Invitrogen). .. The codon-optimized nucleotide sequence for hybrid αβTCR sequences was synthesized (Twist Biosciences) as a single polyprotein separated by a 2A self-cleaving peptide sequence (TCRβ-P2A-TCRα) and subsequently cloned into the pDONR22 entry vector and then transferred into the pHAGE-EF1:DEST-PGK:CD4 destination vector provided by Dr. Mohammad Haj Dezfullian (University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA), using LR Clonase (Invitrogen) according to the Gateway cloning methodology (Invitrogen) and as described previously , and used to transduce 2T1R-competent cells (Invitrogen).
Article Title: Human CD4+ T cells recognize Mycobacterium tuberculosis-infected macrophages amid broader responses.
Article Snippet: .. The codon-optimized nucleotide sequence for hybrid αβTCR sequences was synthesized (Twist Biosciences) as a single polyprotein separated by a 2A self-cleaving peptide sequence (TCRβ-P2A-TCRα) and subsequently cloned into the pDONR22 entry vector and then transferred into the pHAGE-EF1:DEST-PGK: CD4 destination vector provided by Dr. Mohammad Haj Dezfullian (University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA), using LR Clonase (Invitrogen) according to the Gateway cloning methodology (Invitrogen) and as described previously (Dezfulian et al., 2023), and used to transduce 2T1R-competent cells (Invitrogen). .. The codon-optimized nucleotide sequence for hybrid αβTCR sequences was synthesized (Twist Biosciences) as a single polyprotein separated by a 2A self-cleaving peptide sequence (TCRβ-P2A-TCRα) and subsequently cloned into the pDONR22 entry vector and then transferred into the pHAGE-EF1:DEST-PGK: CD4 destination vector provided by Dr. Mohammad Haj Dezfullian (University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA), using LR Clonase (Invitrogen) according to the Gateway cloning methodology (Invitrogen) and as described previously (Dezfulian et al., 2023), and used to transduce 2T1R-competent cells (Invitrogen).
Cloning:Article Title: Human CD4 + T cells recognize Mycobacterium tuberculosis –infected macrophages amid broader responses
Article Snippet: All TCRs were engineered as hybrid TCRs, in which the entire human α and β V(D)J regions were preserved, and these nucleotides were grafted onto mutant mouse α and β constant regions, respectively, and cloned into the pDONR221 Entry vector as described previously ( ). .. The codon-optimized nucleotide sequence for hybrid αβTCR sequences was synthesized (Twist Biosciences) as a single polyprotein separated by a 2A self-cleaving peptide sequence (TCRβ-P2A-TCRα) and subsequently cloned into the pDONR22 entry vector and then transferred into the pHAGE-EF1:DEST-PGK:CD4 destination vector provided by Dr. Mohammad Haj Dezfullian (University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA), using LR Clonase (Invitrogen) according to the Gateway cloning methodology (Invitrogen) and as described previously , and used to transduce 2T1R-competent cells (Invitrogen). .. The codon-optimized nucleotide sequence for hybrid αβTCR sequences was synthesized (Twist Biosciences) as a single polyprotein separated by a 2A self-cleaving peptide sequence (TCRβ-P2A-TCRα) and subsequently cloned into the pDONR22 entry vector and then transferred into the pHAGE-EF1:DEST-PGK:CD4 destination vector provided by Dr. Mohammad Haj Dezfullian (University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA), using LR Clonase (Invitrogen) according to the Gateway cloning methodology (Invitrogen) and as described previously , and used to transduce 2T1R-competent cells (Invitrogen).
Article Title: Human CD4+ T cells recognize Mycobacterium tuberculosis-infected macrophages amid broader responses.
Article Snippet: .. The codon-optimized nucleotide sequence for hybrid αβTCR sequences was synthesized (Twist Biosciences) as a single polyprotein separated by a 2A self-cleaving peptide sequence (TCRβ-P2A-TCRα) and subsequently cloned into the pDONR22 entry vector and then transferred into the pHAGE-EF1:DEST-PGK: CD4 destination vector provided by Dr. Mohammad Haj Dezfullian (University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA), using LR Clonase (Invitrogen) according to the Gateway cloning methodology (Invitrogen) and as described previously (Dezfulian et al., 2023), and used to transduce 2T1R-competent cells (Invitrogen). .. The codon-optimized nucleotide sequence for hybrid αβTCR sequences was synthesized (Twist Biosciences) as a single polyprotein separated by a 2A self-cleaving peptide sequence (TCRβ-P2A-TCRα) and subsequently cloned into the pDONR22 entry vector and then transferred into the pHAGE-EF1:DEST-PGK: CD4 destination vector provided by Dr. Mohammad Haj Dezfullian (University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA), using LR Clonase (Invitrogen) according to the Gateway cloning methodology (Invitrogen) and as described previously (Dezfulian et al., 2023), and used to transduce 2T1R-competent cells (Invitrogen).
Transduction:Article Title: Human CD4 + T cells recognize Mycobacterium tuberculosis –infected macrophages amid broader responses
Article Snippet: All TCRs were engineered as hybrid TCRs, in which the entire human α and β V(D)J regions were preserved, and these nucleotides were grafted onto mutant mouse α and β constant regions, respectively, and cloned into the pDONR221 Entry vector as described previously ( ). .. The codon-optimized nucleotide sequence for hybrid αβTCR sequences was synthesized (Twist Biosciences) as a single polyprotein separated by a 2A self-cleaving peptide sequence (TCRβ-P2A-TCRα) and subsequently cloned into the pDONR22 entry vector and then transferred into the pHAGE-EF1:DEST-PGK:CD4 destination vector provided by Dr. Mohammad Haj Dezfullian (University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA), using LR Clonase (Invitrogen) according to the Gateway cloning methodology (Invitrogen) and as described previously , and used to transduce 2T1R-competent cells (Invitrogen). .. The codon-optimized nucleotide sequence for hybrid αβTCR sequences was synthesized (Twist Biosciences) as a single polyprotein separated by a 2A self-cleaving peptide sequence (TCRβ-P2A-TCRα) and subsequently cloned into the pDONR22 entry vector and then transferred into the pHAGE-EF1:DEST-PGK:CD4 destination vector provided by Dr. Mohammad Haj Dezfullian (University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA), using LR Clonase (Invitrogen) according to the Gateway cloning methodology (Invitrogen) and as described previously , and used to transduce 2T1R-competent cells (Invitrogen).
Article Title: Human CD4+ T cells recognize Mycobacterium tuberculosis-infected macrophages amid broader responses.
Article Snippet: .. The codon-optimized nucleotide sequence for hybrid αβTCR sequences was synthesized (Twist Biosciences) as a single polyprotein separated by a 2A self-cleaving peptide sequence (TCRβ-P2A-TCRα) and subsequently cloned into the pDONR22 entry vector and then transferred into the pHAGE-EF1:DEST-PGK: CD4 destination vector provided by Dr. Mohammad Haj Dezfullian (University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA), using LR Clonase (Invitrogen) according to the Gateway cloning methodology (Invitrogen) and as described previously (Dezfulian et al., 2023), and used to transduce 2T1R-competent cells (Invitrogen). .. The codon-optimized nucleotide sequence for hybrid αβTCR sequences was synthesized (Twist Biosciences) as a single polyprotein separated by a 2A self-cleaving peptide sequence (TCRβ-P2A-TCRα) and subsequently cloned into the pDONR22 entry vector and then transferred into the pHAGE-EF1:DEST-PGK: CD4 destination vector provided by Dr. Mohammad Haj Dezfullian (University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA), using LR Clonase (Invitrogen) according to the Gateway cloning methodology (Invitrogen) and as described previously (Dezfulian et al., 2023), and used to transduce 2T1R-competent cells (Invitrogen).
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